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human igm elisa antibody pair kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc human igm elisa antibody pair kit
    (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) <t>a-CD3/CD28</t> <t>(StemCell).</t> Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by <t>ELISA</t> of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.
    Human Igm Elisa Antibody Pair Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human igm elisa antibody pair kit/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    human igm elisa antibody pair kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node"

    Article Title: Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node

    Journal: bioRxiv

    doi: 10.1101/2025.01.12.632545

    (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) a-CD3/CD28 (StemCell). Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by ELISA of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.
    Figure Legend Snippet: (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) a-CD3/CD28 (StemCell). Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by ELISA of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.

    Techniques Used: Cell Culture, Labeling, Staining, Enzyme-linked Immunosorbent Assay

    (a) Schematic of experimental setup for comparing naïve T versus pre-Tfh cells and presence/absence of SEB on IgM secretion. (b) ELISA data showing IgM secretion, dots represent pooled supernatant from four chips per donor. (c) Schematic of experimental setup for analyzing IgM secretion dependency on proximity between pre-Tfh and activated B cells. (d) Brightfield images showing patterned lymphocytes on chip, day 1. (e) ELISA data showing IgM secretion. Results from four pooled chips from one donor, D68F. Red dotted lines are ELISA limit of detection: 0.140 ng/mL IgM.
    Figure Legend Snippet: (a) Schematic of experimental setup for comparing naïve T versus pre-Tfh cells and presence/absence of SEB on IgM secretion. (b) ELISA data showing IgM secretion, dots represent pooled supernatant from four chips per donor. (c) Schematic of experimental setup for analyzing IgM secretion dependency on proximity between pre-Tfh and activated B cells. (d) Brightfield images showing patterned lymphocytes on chip, day 1. (e) ELISA data showing IgM secretion. Results from four pooled chips from one donor, D68F. Red dotted lines are ELISA limit of detection: 0.140 ng/mL IgM.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    (a) Schematic of experimental setup. (b) Representative composite images (fluorescence overlayed over brightfield) from D69M, cells stained with AF546-anti-CD38 (cyan) on day 7. (c) Change in %CD38 positive area (3 donors). (d) IgM secretion from co-culture, quantified by ELISA on day 6. N=2–3 chips/donor (5 donors). Analyzed using two-way ANOVA with Tukey’s multiple comparisons test.
    Figure Legend Snippet: (a) Schematic of experimental setup. (b) Representative composite images (fluorescence overlayed over brightfield) from D69M, cells stained with AF546-anti-CD38 (cyan) on day 7. (c) Change in %CD38 positive area (3 donors). (d) IgM secretion from co-culture, quantified by ELISA on day 6. N=2–3 chips/donor (5 donors). Analyzed using two-way ANOVA with Tukey’s multiple comparisons test.

    Techniques Used: Fluorescence, Staining, Co-Culture Assay, Enzyme-linked Immunosorbent Assay



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    STEMCELL Technologies Inc human igm elisa antibody pair kit
    (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) <t>a-CD3/CD28</t> <t>(StemCell).</t> Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by <t>ELISA</t> of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.
    Human Igm Elisa Antibody Pair Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human igm elisa antibody pair kit/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    human igm elisa antibody pair kit - by Bioz Stars, 2026-03
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    human igg or igm elisa antibody pair kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc human igg or igm elisa antibody pair kit
    (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) <t>a-CD3/CD28</t> <t>(StemCell).</t> Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by <t>ELISA</t> of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.
    Human Igg Or Igm Elisa Antibody Pair Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human igg or igm elisa antibody pair kit/product/STEMCELL Technologies Inc
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    human igg or igm elisa antibody pair kit - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) a-CD3/CD28 (StemCell). Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by ELISA of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.

    Journal: bioRxiv

    Article Title: Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node

    doi: 10.1101/2025.01.12.632545

    Figure Lengend Snippet: (a-c) Naïve T cells were cultured on chip with IL-7. Representative image (b) and quantification (c) of T cell viability after 4 days, labeled with (Calcein AM, green, and Dapi, blue) for 3 donors. (d-g) A CCL21 gradient was established on chip, with CCL21 added to the left-hand media lane. Representative images (e) and quantification (f) of naive CD4+ T cells after migrating toward CCL21 for 1 hr and staining with Calcein AM (green). (g) Quantification of cell velocity 30 min after gradient set up (cells were unlabeled). (h-k) Naïve T cells were cultured without (i) and with (ii) a-CD3/CD28 (StemCell). Images of CD69+ signal (FITC-anti-CD69, green) for (i) naïve and (ii) activated T cells on-chip, and quantification (j) of CD69 signal after 48 hours (unpaired T test, **:p<0.005). (k) Quantification of IFN-γ secretion by activated or naïve CD4+T cells on-chip measured by ELISA of supernatants collected at day 5. Panels f, g, k analyzed with ordinary two-way ANOVA with Sidak’s multiple comparisons test w/single pooled variance, ns: p>0.05, *:p<0.05, ****:p<0.00005.

    Article Snippet: IgM was detected using a Human IgM ELISA Antibody Pair Kit (StemCell technologies, Cat#01995), and IL-21 was detected using an ELISA Flex: Human IL-21 (HRP) kit (Mabtech, Ohio, USA, Cat#3540–1H-6) following manufacturer protocols.

    Techniques: Cell Culture, Labeling, Staining, Enzyme-linked Immunosorbent Assay

    (a) Schematic of experimental setup for comparing naïve T versus pre-Tfh cells and presence/absence of SEB on IgM secretion. (b) ELISA data showing IgM secretion, dots represent pooled supernatant from four chips per donor. (c) Schematic of experimental setup for analyzing IgM secretion dependency on proximity between pre-Tfh and activated B cells. (d) Brightfield images showing patterned lymphocytes on chip, day 1. (e) ELISA data showing IgM secretion. Results from four pooled chips from one donor, D68F. Red dotted lines are ELISA limit of detection: 0.140 ng/mL IgM.

    Journal: bioRxiv

    Article Title: Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node

    doi: 10.1101/2025.01.12.632545

    Figure Lengend Snippet: (a) Schematic of experimental setup for comparing naïve T versus pre-Tfh cells and presence/absence of SEB on IgM secretion. (b) ELISA data showing IgM secretion, dots represent pooled supernatant from four chips per donor. (c) Schematic of experimental setup for analyzing IgM secretion dependency on proximity between pre-Tfh and activated B cells. (d) Brightfield images showing patterned lymphocytes on chip, day 1. (e) ELISA data showing IgM secretion. Results from four pooled chips from one donor, D68F. Red dotted lines are ELISA limit of detection: 0.140 ng/mL IgM.

    Article Snippet: IgM was detected using a Human IgM ELISA Antibody Pair Kit (StemCell technologies, Cat#01995), and IL-21 was detected using an ELISA Flex: Human IL-21 (HRP) kit (Mabtech, Ohio, USA, Cat#3540–1H-6) following manufacturer protocols.

    Techniques: Enzyme-linked Immunosorbent Assay

    (a) Schematic of experimental setup. (b) Representative composite images (fluorescence overlayed over brightfield) from D69M, cells stained with AF546-anti-CD38 (cyan) on day 7. (c) Change in %CD38 positive area (3 donors). (d) IgM secretion from co-culture, quantified by ELISA on day 6. N=2–3 chips/donor (5 donors). Analyzed using two-way ANOVA with Tukey’s multiple comparisons test.

    Journal: bioRxiv

    Article Title: Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node

    doi: 10.1101/2025.01.12.632545

    Figure Lengend Snippet: (a) Schematic of experimental setup. (b) Representative composite images (fluorescence overlayed over brightfield) from D69M, cells stained with AF546-anti-CD38 (cyan) on day 7. (c) Change in %CD38 positive area (3 donors). (d) IgM secretion from co-culture, quantified by ELISA on day 6. N=2–3 chips/donor (5 donors). Analyzed using two-way ANOVA with Tukey’s multiple comparisons test.

    Article Snippet: IgM was detected using a Human IgM ELISA Antibody Pair Kit (StemCell technologies, Cat#01995), and IL-21 was detected using an ELISA Flex: Human IL-21 (HRP) kit (Mabtech, Ohio, USA, Cat#3540–1H-6) following manufacturer protocols.

    Techniques: Fluorescence, Staining, Co-Culture Assay, Enzyme-linked Immunosorbent Assay